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KMID : 0857019990140010001
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Kosin Medical Journal
1999 Volume.14 No. 1 p.1 ~ p.10
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The Effect of Direct Current Electric Stimulation on the Regeneration of Sciatic Nerve after Neurorrhaphy in Rabbit
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Kim Jae-Do
Moon Yong-Sik
Kwon Young-Ho
Lee Kyu-Yong
Jeong Ho-Joong
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Abstract
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Background : Although the application of d.c. electric current has been proposed as a means to improve peripheral nerve regeneration, its usefulness has not been firm established. The present study was, therefore, undertaken to reevaluate the effect of d.c. current on the regeneration of peripheral nerve and to determine if intraoperative stimulation and recording technique is practical in clinics.
Method : Sciatic nerves of rabbits were transected and sutured with 8-0 prolene. The sutured nerves were then either treated with 15μA d.c. current for 1, 2, or 3 weeks (Neurorrhaphy + d.c. group) or maintained without any treatment (Neurorrhaphy group). At one-week intervals after operation, compound muscle action potentials and intraoperative compound nerve action potentials were recorded in each group to evaluate the functional recovery of the nerve. At the end of third week experiments, sciatic nerve samples were prepared for histologic examination of regeneration.
Result : There exited a significant positive correlation between the onset latencies of intraoperative nerve action potenial and compound muscle action potential(r=0.68, p=0.003). Histologic examination indicated that sciatic nerve of the Neurorrhapy +d.c. 1-week group contain less vacuoles than those in controls and the jumber of myelinated fibers in the Neurorrhapy+d.c. 1-week group(1685±321) was significantly(p<0.05) higher than that in the Neurorrhapy group after 3 weeks(1324±374). The results of the study demonstrated that while excellent regeneration occurred on the sciatic nerve treated with d.c. electric field for 1 week, the long application of d.c. electric fields could not support the regeneration of peripheral nerve.
Conclusion : Such results indicate that short applied d.c electric field might decrease the latency period, or the extent of retrograde dieback. These results suggested that intraoperative use of stimulating and recording technique was practical and useful.
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KEYWORD
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Nerve regeneration, d.c. electric stimulation, Intraoperative compound nerve action potential
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